Evaluation of influenza RT-PCR assay competency
نویسندگان
چکیده
منابع مشابه
Detection of Seasonal Influenza H1N1 and H3N2 Viruses using RT-PCR Assay during 2009 Flu Pandemic in Golestan Province
Abstract Background and Objective: The emergence of a novel H1N1influenza A virus of animal origin with transmissibility from human to human poses pandemic concern. Current subtypes of Seasonal influenza A viruses spread in human are influenza A H1N1 influenza A H3N2 and influenza type B viruses. The aim of this study was to determine current strains of the H3N2 and new H1N1 subtypes of influe...
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The PLEX-ID/Flu assay has been recently developed to enable the detection and typing of influenza viruses based on the RT-PCR/electrospray ionization mass spectrometry technology.This novel assay was evaluated for typing performance on 201 positive influenza A or B nasopharyngeal swab specimens (NPS) detected by real-time RT-PCR during the 2010-2011 season. The PLEX-ID/Flu assay detected and ch...
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BACKGROUND Influenza C virus can cause both upper and lower respiratory tract infections and has been reported to be prevalent in children. However, these infections have been under-diagnosed, and epidemiological data available are limited due to the lack of convenient detection assays. OBJECTIVE Design and validate a real-time reverse-transcriptase PCR (rt RT-PCR) assay for the detection of ...
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Influenza virus infections have been causing major public health concerns worldwide. Therefore, rapid and accurate diagnostic methods for typing and subtyping of influenza virus are crucial both for patient management and to limit dissemination. In this study, multiplex RT-PCR was developed, optimized and evaluated for detection, typing and subtyping of influenza viruses. The typing assay consi...
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For surveillance purposes real-time PCR assays for influenza viruses had to be adapted to the pandemic influenza A(H1N1)2009 strain. We combined published primers and probes for influenza A, influenza B and an internal amplification control with a detection system for influenza A(H1N1)2009 to set up a rapid, reliable, simple and cost-effective high-throughput multiplex one-step real-time RT-PCR...
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ژورنال
عنوان ژورنال: International Journal of Infectious Diseases
سال: 2012
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2012.05.682